FIG. 5.

VDR-mediated transactivation stimulated by OCT is potentiated by TIF2 but not by SRC-1. The effects of SRC-1 (upper panel) and TIF2 (lower panel) on the AF-2 of VDR bound to vitamin D analog were examined in COS-1 cells. The CAT assay was performed with a reporter plasmid (17M2-G-CAT) and pM(GAL4-DBD)-VDR(DEF), pM(GAL4-DBD)-VDR(L417S), or pM(GAL4-DBD)-VDR(E420Q) mutant expression vector with or without an expression plasmid of the SRC-1/TIF2 protein family, as described in the legend for Fig. 1. Values are expressed as means ± standard deviations of triplicate transfections.