Table 4. Haematological variables.
| Haematological profile | Analytical techniques |
| Complete blood count | Automatic cell counter: XN-10/XN-20 (Sysmex) |
| Erythrocyte sedimentation rate | Westergren method: Ves-matic Cube 30 (Menarini) |
| Haemostasis/fibrinolysis | Analytical techniques |
| Prothrombin time | Clot-based assay: ACLTOP 750 (Werfen) |
| Partial activated thromboplastin time | |
| Fibrinogen (coagulative) | Clot-based assay, Clauss method: ACLTOP 750 (Werfen) |
| Biochemical variables | Analytical techniques |
| Glucose | Spectrophotometry, enzyme assay: Alinity c (Abbott) |
| Urea | |
| Uric acid | |
| Cholesterol | |
| HDL cholesterol | |
| Triglycerides | |
| Lactate dehydrogenase | |
| Gamma glutamyltransferase | |
| Aspartate transaminase | |
| Alanine transaminase | |
| Creatine kinase | |
| Alkaline phosphatase | |
| Albumin | Spectrophotometry, colorimetric assay: Alinity c (Abbott) |
| Calcium | |
| Phosphorus | |
| Iron | |
| Creatinine | Spectrophotometry modified Jaffé method: Alinity c (Abbott) |
| Alpha-amylase | Spectrophotometry, enzyme/colourimetric assay: Alinity c (Abbott) |
| LDL cholesterol (calculated) | Friedewald equation |
| Bilirubin total | Spectrophotometry, diazo reaction: Alinity c (Abbott) |
| Bilirubin direct | |
| Specific proteins | Analytical techniques |
| C reactive protein | Spectrophotometry, immunoturbidimetry assay: Alinity c (Abbott) |
| Interleukin 6 | Immunochemistry, sandwich principle: Cobas e402 (Roche) |
| Ferritin | Chemiluminescence: Alinity i (Abbott) |
| Hormones | Analytical techniques |
| Thyrotropin | Chemiluminescence: Alinity i (Abbott) |
| Somatotropin | Chemiluminiscence: Maglumi 2000 Plus (Snibe) |
| Cortisol | Chemiluminescence: Alinity i (Abbott) |
| Vitamins | Analytical techniques |
| Vitamin D (25 hydroxy) | Chemiluminescence: Alinity i (Abbott) |
| Immunological profile | Analytical techniques |
| Adiponectin | Immunoassay: LUMINEX 200 System (Bio-Rad Laboratories) |
| Complement factor C3 | Nephelometry: IMMAGE 800 (Beckman Coulter) |
| Complement factor C4 | |
| Human Neuropeptide Y | ELISA: GENLISA, Varioskan LUX multimode microplate (Thermo Fisher) |
| Substance P | |
| Human gamma-aminobutyric acid (GABA) | |
| Human Cystatin C | ELISA: CST3 ELISA Kit PicoKine, Varioskan LUX multimode microplate (Thermo Fisher) |
| Human tumour necrosis factor-alpha | ELISA: TNF ELISA Kit PicoKine, Varioskan LUX multimode microplate (Thermo Fisher) |
| Interferon gamma | Immunoassay: LUMINEX 200 System (Bio-Rad Laboratories) kit LUMINEX HU ACDC SIMPLEX |
| IL-1 alpha | Immunoassay: LUMINEX 200 System (Bio-Rad Laboratories) |
| IL-1 beta | |
| IL-8 (CXCL8) | |
| IL-10 | |
| Leptin | |
| Matrix metalloproteinase-1 | |
| Nerve growth factor beta |
CXCL8motif chemokine ligand 8HDLhigh-density lipoprotein cholesterolILinterleukinLDLlow-density lipoprotein cholesterol