Table 2. Antibodies.
| Antigen | Immunogen | Host/type | Manufacturer | Catalog # | RRID # | Dilution |
|---|---|---|---|---|---|---|
| OTP | Recombinant protein [DPGGHPGDLAPNSDPVEGATC] | Guinea Pig, polyclonal | Jessell Lab/ HHMI CU | CU1497 | RRID:AB_2665423 | 1:3000 |
| OTP | Recombinant protein (aa1–130 from human OTP) | Rabbit, polyclonal | Thermo Fisher | PA5-89060 | RRID:AB_2805328 | 1:2000 |
| Pou6f2 | Recombinant protein (Pou6f2) | Rabbit, polyclonal | Sigma | hpa008699 | RRID:AB_1079664 | 1:1000 |
| Pou6f2 | Synthetic peptide amino acids 35–184 of human Pou6f2 | Guinea Pig, polyclonal | Jessell Lab/ HHMI CU | RRID:AB_2665423 | 1:500 | |
| Pou6f2 | Synthetic peptide amino acids 35–184 of human Pou6f2 | Rat, monoclonal | Jessell Lab/ HHMI CU |
RRID:AB_2665427 | 1:1000 | |
| Sp8 | Synthetic peptide (C-terminus [C-18] from human Sp8) | Goat, polyclonal | Santa Cruz | sc-104661 | RRID:AB_2194626 | 1:2000 |
| Foxp2 | Synthetic peptide (N-terminus [N-16] from human FOXP2) | Goat, polyclonal | Santa Cruz | sc-21069 | RRID:AB_2107124 | 1:2000 ICC and WB |
| Foxp4 | Recombinant protein [DPGGHPGDLAPNSDPVEGATC] | Rabbit, polyclonal | Jessell Lab/ HHMI CU |
CU1464 | RRID:AB_2665415 | 1:25000 |
| MafA | Recombinant protein (aa300–359 [C-terminus] from mouse v-mafA) | Rabbit, polyclonal | Novus | NB400-137 | RRID:AB_10002142 | 1:500 WB |
| MafB | Recombinant protein (Transcription factor MafB) | Rabbit, polyclonal | Sigma | hpa005653 | RRID:AB_1079293 | 1:1000 ICC and WB |
| MafB | Recombinant protein (aa100–150 from mouse MafB) | Rabbit, polyclonal | Novus | NB600-266 | RRID:AB_2137664 | 1:250 ICC 1:500 WB |
| c-Maf | Recombinant protein (aa150–200 from mouse c-Maf) | Rabbit, polyclonal | Novus | NB600-267 | RRID:AB_2137514 | 1:500 WB |
| NeuroD2 | Synthetic peptide Mouse NeuroD2 aa1–100 conjugated to KLH | Rabbit, polyclonal | AbCam | Ab104430 | RRID:AB_10975628 | 1:2000 |
| Prox1 | Synthetic peptide from the C-terminus of mouse Prox1 | Rabbit, polyclonal | Millipore Sigma | AB5475 | RRID:AB_177485 | 1:500 |
| RFP | Recombinant protein (RFP from Discosoma sp.) | Mouse, monoclonal | Rockland | 200-301-379 | RRID:AB_2611063 | 1:1000 |
| dsRed | Recombinant protein (RFP variant from Discosoma sp.) | Rabbit, polyclonal | Clontech | 632496 | RRID:AB_10013483 | 1:1000 |
| GFP | Recombinant protein (GFP from Aequorea victoria) | Chicken, polyclonal | Aves | GFP-1020 | RRID:AB_10000240 | 1:1000 |
| ChAT | Human placental enzyme | Goat, polyclonal | EMD Millipore | AB144P | RRID:AB_2079751 | 1:100 |
| NeuN | Synthetic peptide of human RBFOX3/NeuN protein (aa20–100) | Rabbit, polyclonal | Novus | NBP1-77686 | RRID:AB_11009597 | 1:1000 |
| NeuN | Isolated brain cell nuclei | Mouse clone A60 | Millipore | MAB377 | RRID:AB_2298772 | 1:100 |
| Calbindin | Recombinant protein (calbindin D-28k from rat) | Rabbit, polyclonal | Swant | CB-38a | RRID:AB_10000340 | 1:1000 |
| VGLUT1 | Synthetic peptide (aa456–560 from rat VGLUT1) | Guinea Pig/ polyclonal | Synaptic Systems | 135-304 | RRID:AB_887878 | 1:1000 |
| VGAT | Synthetic peptide (N-terminus from rat VGAT) | Mouse/ monoclonal | Synaptic Systems | 131-011 | RRID:AB_887872 | 1:100 |
| VGAT | Recombinant protein (N-terminus from rat VGAT) | Guinea Pig/ polyclonal | Synaptic Systems | 131-004 | RRID:AB_887873 | 1:200 |
| CTB | Cholera toxin B subunit | Goat/ polyclonal | List Labs | #703 | RRID:AB_10013220 | 1:200 |
| acetyl-Histone H3 | Linear peptide from human Histone H3 acetylated at the N-terminus | Rabbit/ polyclonal | EMD Millipore | 06-599 | RRID:AB_2115283 | 1:1000 |