FIG. 2.
(A) Physical interaction between Rad17 and Mec3 in vivo. Extracts were prepared from rad17Δ mec3Δ (KSC1085) cells transformed with YCpT-RAD17-myc, YCpMEC3-HA, or the vectors and subjected to immunoprecipitation (IP) with anti-Myc (α-myc) or anti-HA (α-HA) antibody. The immunoprecipitates were separated by SDS-PAGE and subjected to immunoblotting analysis with anti-Myc or anti-HA antibody. (B) Failure of Rad17E128K to interact physically with Mec3. rad17Δ mec3Δ (KSC1085) cells carrying YCpMEC3-HA were transformed with YCpT-RAD17-myc or YCpT-RAD17(E128K)-myc. Extracts were prepared from the transformants and subjected to immunoprecipitation with anti-Myc antibody. The extracts and immunocomplexes were separated by SDS-PAGE and immunoblotted with anti-HA or anti-Myc antibody.