Figure 6.

No Rescue of Cardiac Morphology via Cardiomyocyte-Specific Lamin-A Addback

(A) A diagram showing the design of cardiomyocyte-specific lamin-A addback experiments. (B) Representative fluorescence images of AAV-treated cardiomyocytes and quantification of percentages of GFP-LA-–positive cardiomyocytes. Bar = 20 μm. Student’s t-test; NS. (C) Growth curve of animals in the cardiomyocyte-specific lamin-A addback experiments. (D) Echocardiogram analysis of the 6-week-old mice. (E,F) Quantification of morphology of isolated cardiomyocytes (E) or cells in heart sections (F). Bar = 20 μm. (E,F) Cells from littermates were compared. (G) A diagram showing cardiomyocyte-specific lamin-A addback in a genetic mosaic fashion. (H) Fluorescence imaging of isolated cardiomyocytes from the mosaic hearts. Cell borders was annotated with dashed lines. Arrows indicate GFP-LA–positive nuclei. Bar = 10 μm. (I,J) Quantification of cardiomyocyte (I) and nuclear (J) morphology. (D to F,I,J) Mann-Whitney U test; ∗P < 0.05, NS. Abbreviations as in Figures 2, 3, and 5.