Figure 1.

Characterization and cloning of P63⁺ progenitor cells from patients with bronchiectasis

(A) Immunofluorescence staining showing KRT5⁺ (red) cells in lung sections from different patients with bronchiectasis (n = 5). Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm.

(B) A schematic diagram illustrating the manufacture, quality control, and clinical administration procedure of autologous P63⁺ progenitor cell products.

(C) Cultured progenitor cell clones were immunostained with KRT5 and P63 markers. Scale bar, 100 μm.

(D) Fluorescence-activated cell sorting (FACS) gating strategy for cell identity and purity test. KRT5 was immunostained as a marker of progenitor cells. SSC, side-scatter; FSC, forward scatter.