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. 2024 Nov 28;15:10332. doi: 10.1038/s41467-024-54580-9

Fig. 3. Penetration of infused VRC01 and VRC01LS into the male intestinal compartment and the lower FGT.

Fig. 3

Protein-normalized mAb levels of (a) VRC01 and (b) VRC01LS in male participants who received a single IV infusion at 30 mg/kg. Blood serum (yellow diamonds), rectal tissue lysates (red triangles), clarified Seminal Fluid (purple asterisks), and clarified rectal secretions (blue crosses) are depicted. Gray dashed lines denote each individual participant, and colored bold lines represent the median of each group. Fourteen of the rectal secretions (n = 4; 21.5% of collections) were excluded due to high hemoglobin, implying blood contamination. Eight rectal secretions had insufficient quantity for protein quantitation and were excluded. Three rectal secretions and two rectal biopsy lysates were below the Quant-iT LLOQ, so their denominator was replaced by ½ the LLOQ of their QuantiT runs. c Percent penetration in tissues and secretions from male participants. Protein-normalized concentrations of VRC01 and VRC01LS in female participants who received (d) VRC01 or (e) VRC01LS. Median serum (yellow diamonds), cervical biopsy lysates (blue circles), vaginal biopsy lysates (black squares), and cervicovaginal secretions (green triangles) are depicted. Gray dashed lines denote each individual participant, and colored bold lines represent the median of each group. Seven cervicovaginal secretions (n = 6 females; 8.9% of collections) did not contain sufficient fluid to assay, and 7 cervicovaginal secretions (n = 5; 8.9% collections) had evidence of hemoglobin contamination; these were excluded. Two cervical biopsy lysates and four vaginal biopsy lysates did not have sufficient protein for quantitation, so their denominator was replaced by ½ the LLOQ of their QuantiT runs. f Percent penetration in tissues and secretions from female participants. The p-values in (c and f) are two-sided Wilcoxon ranked sum tests adjusted for multiple comparisons using the Holm-Bonferroni method. Abbreviations: mAb monoclonal antibody, IQR inter-quartile range, NC not calculated.