Fig. 1.

Dissected brainstem regions and differential expression analyses. a–c) overview schematic of regions microdissected in the midbrain dorsal raphe, medullary raphe, and ventrolateral medulla. After proteomic analysis, principal component analysis (PCA) shows distribution of SUDC cases with febrile seizure history (SUDC-FS; orange) and SUDC cases without febrile seizure history (SUDC-noFS; yellow) in the midbrain dorsal raphe d), medullary raphe e), and ventrolateral medulla f). There was no segregation of cases by FS history in any of the brain regions analyzed, in PCA1: midbrain dorsal raphe (p = 0.93, unpaired t-test), medullary raphe (p = 0.12), ventrolateral medulla (p= 0.24) or PCA2: midbrain dorsal raphe (p = 0.055), medullary raphe (p = 0.33), ventrolateral medulla (p = 0.72). g) Differential expression analysis identified 178 altered proteins (68 increased, 110 decreased) in the midbrain dorsal raphe at p < 0.05. h In the medullary raphe, 344 proteins were altered (217 increased, 127 decreased). i In the ventrolateral medulla, 100 proteins were altered (53 increased, 47 decreased). The top 5 significantly increased and decreased proteins are annotated by gene name, as well as protein of interest SLC2A13. Dotted lines correspond to p < 0.05 and fold change at 1.5. Cell type annotation for each protein is indicated by color. Additional protein information is available in Supplementary Tables 2, 3, 4