Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Nov 28;148(1):76. doi: 10.1007/s00401-024-02832-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

PMC Copyright notice

Fig. 4 — Histological localization and quantification of top protein candidates SLC2A13 and SEZ6L2 in the brainstem. a By LC–MS/MS in the midbrain dorsal raphe, SLC2A13 was among the top significant proteins and had the largest fold change with a 24.9-fold decrease in SUDC-FS when compared to SUDC-noFS (p = 3.97 × 10^–3), b IF for SLC2A13 in the midbrain dorsal raphe showed a similar trend with a 1.5-fold decrease in SUDC-FS (p = 0.23) from semiquantitative analysis, c IF for TPH2 on the same slides for SLC2A13 in the midbrain dorsal raphe showed no difference between SUDC-FS and SUDC-noFS, similar to proteomics, d colocalization analysis of SLC2A13 and TPH2 in the midbrain dorsal raphe showed a moderate correlation by Mander coefficient of the green channel (TPH2), which was different between SUDC-FS and SUDC-noFS (p = 0.026). There was a higher correlation in the red channel (SLC2A13) for both SUDC-FS and SUDC-noFS. e By LC–MS/MS in the medullary raphe, SEZ6L2 was among the top significant proteins and had the largest fold change with a 3.9-fold increase in SUDC-FS (p = 4.75 × 10^–5), f IF for SEZ6L2 in the medullary raphe showed a similar trend with a 1.3-fold increase (p = 0.49) from semiquantitative analysis, g IF for TPH2 on the same slides for SEZ6L2 in the medullary raphe showed no difference between SUDC-FS and SUDC-noFS, similar to proteomics, h colocalization analysis of SEZ6L2 and TPH2 in the medullary raphe showed a low correlation in the red channel and moderate correlation in the green channel, which was not different between SUDC-FS and SUDC-noFS, i representative images from IF in the midbrain dorsal raphe are shown for SLC2A13 (red) and TPH2 (green) in SUDC-FS and SUDC-noFS. TPH2 indicates the region with serotonergic neurons that was microdissected for proteomic analysis. SLC2A13 was present in TPH2( +) cells, as well as in other neighboring TPH2(−) cells, j representative images from IF in the medullary raphe are shown for SEZ6L2 (red) and TPH2 (green) in SUDC-FS and SUDC-noFS. TPH2 indicates the region with serotonergic neurons that was microdissected for proteomic analysis. SEZ6L2 was present in TPH2( +) cells, as well as in other neighboring TPH2(−) cells. Scale bar 100 um. Error bars indicate SEM