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Subcellular localization of Rex proteins in transfected HeLa cells. Monolayers were transiently transfected with the wild-type vector pcRex (a and b) or its mutated derivative pcRex (1 + 2) (c and d), maintained for 48 h and subjected to indirect immunofluorescence using a Rex-specific antiserum raised in rabbit and a Texas red-conjugated secondary antibody. Samples were viewed by epifluorescence (a and c) or phase contrast (b and d).
