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Inhibition of nuclear import by wheat germ agglutinin. In vitro import assays using GST-Rex NLS (wild type) (a, c, and e) or GST-T NLS (b, d, and f) as substrate and HeLa cell cytosol were carried out in the presence of 0.5 mg of WGA/ml (a and b) or 0.05 mg of WGA/ml (c and d), or in the absence of WGA (e and f). All samples were analyzed by indirect immunofluorescence as described in the legend for Fig. 3.
