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. 2024 Nov 28;7:1587. doi: 10.1038/s42003-024-07278-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a A phylogenetic network, based on 695,229 genome-wide SNPs, showing uncorrected p-distances between 222 isolates of the L. braziliensis clade (incl. L1, L3, and L. peruviana). b, c Principal component analysis for the 222 isolates showing the first three PC axes. d–f ADMIXTURE bar plots showing the estimated ancestry per isolate assuming K = 2 (d), K = 3 (e), and K = 4 (f) ancestral components. g Sample size distribution of Leishmania isolates from each group and per ecoregion. The four colours match the four ancestral components as inferred with ADMIXTURE K = 4 (f). Only isolates with at least 70% ancestry for a specific ancestral component were included. Ecoregion data is available from: https://gaftp.epa.gov/EPADataCommons/ORD/Ecoregions/sa/.