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4% (w/v) Paraformaldehyde (PFA)
| Reagent | Final concentration | Amount |
|---|---|---|
| Paraformaldehyde (PFA) | 1.3 mM | 10 g |
| 2.5N sodium hydroxide solution | 10 mM | 1.5 L |
| 0.2 M Na2HPO4 (dibasic) | 68 mM | 85 mL |
| 0.2 M NaH2PO4 (monobasic) | 32 mM | 40 mL |
| ddH2O | N/A | 125 mL |
| Total | N/A | ∼250 mL |
