Figure 4.
Dose-response rate and antigen breadth after immunization with nucleoside-modified Gag mRNA-LNPs and galsomes
C57BL/6 mice were immunized with 0.5, 2, or 5 μg ψ/m5C-modified Gag HxB2 mRNA encapsulated in LNP (orange) or galsomes (GAL) (blue) following a prime-boost regimen with a 2-week interval. Five days after boost immunization, spleens were collected and splenocytes were analyzed via ELISPOT for IFN-γ secretion after stimulation with a Gag-peptide pool consisting of 113 overlapping peptides. (A) For dose determination, mice were immunized with 3 different doses, as indicated on the graph. (B) Summary of the mice receiving either the initial mRNA-LNP or mRNA-galsomes formulation (fresh) or the frozen formulation (stored), summary of 2 experiments, with n = 8 for immunized mice, n = 6 for PBS controls. Median values are displayed on the graph, Mann-Whitney U test, p value shown on graph.