Figure 3.

The landscape of downstream effects

(A) The statistical model used to relate the 84 perturbed genes to the expressed genes.

(B) The distribution of the number of downstream effects for each of the 84 genes, stratified by gene group. Genes that are outliers with respect to their gene group distribution are labeled.

(C) The distribution of indegree for each of the non-perturbed genes. Outlier genes are labeled.

(D) Association between the properties of downstream genes and the gene set of the upstream regulators. Coefficients are estimated with negative binomial regressions of the gene-set-specific indegree. Downstream gene annotations are indicated on the y axis, and the facets are used to indicate the gene set of the upstream regulator. ∗The 306 denotes the total number of RNA-seq observations, which includes 84 genes perturbed in three donors and 54 samples from control guides.