FIG. 7.

p53-mediated repression of AFP is hepatoma specific. NIH 3T3 cells were transfected with wild-type or mutant p53 expression vector at 1× or 2× concentrations as indicated (3 or 6 μg/plate, respectively) and/or HNF-3 expression vector (3 μg/plate), in addition to AFP/lacZ (3 μg/plate). All plates contain 1 μg of pGL2 luciferase reporter as an internal control. Averaged values are expressed as the fold activation above normalized AFP/lacZ β-galactosidase activity cotransfected with carrier DNA only. Experiments were performed in duplicate or triplicate at least twice.