Fig. 6.

Knockdown of UBR5 alleviates ROS production and senescence in HUVECs. A–E HUVEC-Flag and HUVEC-Flag-TERT cells were transiently transfected with siUBR5 or siNC and treated with 100 μM H₂O₂ for 6 h. A Relative UBR5 mRNA levels in HUVECs were measured by RT-qPCR. B Cells were subjected to SA-β-Gal staining; the percentage of SA-β-Gal-positive cells was quantified from five randomly selected fields. Scale bars, 500 μm. C EdU-labeled cells were stained with anti-EdU (red), and nuclei were counterstained with Hoechst 33342 (blue). The percentage of EdU-positive cells was quantified from five randomly selected fields. Scale bars, 150 μm. D Cells were stained with MitoSOX Red; nuclei were labeled with DAPI (blue). Scale bars, 30 μm. The relative MitoSOX fluorescence intensity was measured from five randomly selected fields. E Relative mtDNA content in cells was determined by RT-qPCR. (ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001)