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. 2024 Nov 29;25:1157. doi: 10.1186/s12864-024-11013-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — csRNA-seq facilitates improved genome annotations. (A) Comparison of experimentally defined TSSs from human BT474 cancer cells, (B) cattle, and (C) bison by csRNA-seq sequenced using AVITI relative to the RefSeq annotation. (D) Comparison of the frequency of TATA box sites per 1000 bp between our experimental TSS and RefSeq for human, (E) cattle, and (F) bison. (G) Comparison of the frequency of Initiator sites per 1000 bp between our experimental TSS and RefSeq for human, (H) cattle, and (I) bison