Fig. 2.

Comparison of circulation metabolic behavior of albumin-seeking dyes in vivo. (a) Serum was collected at time points after tail vein injection (50 µM, 60 µL) of IR-780, IR-808, IR-783 and ICG and imaged under the NIR-II window (80 ms, 1100 LP). (b) Normalized fluorescence intensity of serum after injection of dyes (mean ± SEM, n = 3 for each group). (c) Gel electrophoresis analysis of dyes and albumin binding changed over time in mice serum. (d) Coomassie brilliant blue showed protein distribution of serum after gel electrophoresis. (e) Normalized fluorescence intensity of albumin complex band of IR-780@albumin, IR-808@albumin, and IR-783@albumin and free ICG (mean ± SEM, n = 3 for each group)