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(A) LY294002 (LY, 25 μM) and wortmannin (WM, 300 nM), but not rapamycin (Rap, 30 nM) abrogate the effects of bFGF (10 ng/ml), PDBu (10−7 M) or serum (5% [vol/vol]) on upregulation of cyclin D1 protein. (B) bFGF- or PDBu-induced expression of p21Waf1/Cip1 is resistant to LY294002. (C) Upregulation of the cyclin D1 mRNA by bFGF, PDBu, or serum is abolished by LY294002 but not rapamycin. The cells were stimulated by either of the growth stimuli for 9 h.
