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. 2024 Aug 23;22(12):3244–3261. doi: 10.1111/pbi.14445

Figure 8.

Figure 8

MdNAC7 does not directly regulates MdXTH2 expression, but the protein complex formed with MdWRKY31 hinders MdWRKY31 from binding to the promoter of MdXTH2. (a) EMSA experiments show that the MdNAC7 protein not binds to the MdXTH2 promoter. Lane 1, EMSA with MdNAC7 protein; Lane 2, EMSA with MdNAC7 protein and biotin probe. (b) Relative LUC activity at different injection sites. Representative images of N. benthamiana leaves 3 days after infiltration. (c) Quantitative statistics of fluorescence intensity. 62SK + LUC as negative control. Means and standard deviations were calculated from the results of and three technical repetitions. Error bars indicate technical triplicates with error bars reflecting SD. P < 0.05 by one‐way ANOVA analysis. Different letters indicate significant difference. (d) EMSA experiments show that interaction of MdNAC7 with MdWRKY31 hinders MdWRKY31 from binding to the MdXTH2 promoter. In the context of co‐incubation of MdWRKY31 protein with MdXTH2 biotin probe, MdNAC7 protein was added and the binding bands disappeared.