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(A) Sixteen designs were selected on the basis of in silico site mutagenesis toward AA and 6-ACA. (B) UAA/U6-ACA of the 16 designs and WT. Detailed activity toward 6-ACA and AA were shown in fig. S7. (C) Conversion of AA to 6-ACA using purified MabCAR3_G418K and other necessary enzymes.
