Table 2. Comparison for HMD biosynthesis.
ADH, alcohol dehydrogenase; CHMO, Baeyer-Villiger cyclohexanone monooxygenase; Lact, lactonase; CAR, carboxylic acid reductase; AKR, aldo-keto reductase; ω-TA, ω-transaminase; Cyp, cytochrome P450 monooxygenase. N/A, not describe in the paper.
| Substrate | Catalyst | Titer (mM) | Productivity (g liter−1 hour−1) | |
|---|---|---|---|---|
| Petroleum-based | Cyclohexanol | ADH, CHMO, Lact, CAR, AKR, and ω-TA | 16.6 | 0.088 (56) |
| Cyclohexanol | ADH, CHMO, Lact, CAR, AKR, and ω-TA | 4.2 | N/A (57) | |
| Cyclohexane | Cyp, ADH, CHMO, Lact, CAR, AKR, and ω-TA | 7.6 | 0.040 (56) | |
| Bio-based | AA | CAR (MAB4714 and MAB4714_L342E) and ω-TA | 3.0 | 0.046* (33) |
| AA | CAR (MabCAR3) and ω-TA | 1.0 | 0.005 (32) | |
| AA | CAR (MAB4714 and MAB4714_L342E) and ω-TA | 2.1 | 0.010 (58) | |
| AA | CAR (MabCAR3_L342D) and ω-TA | 11.2 | 0.072 (47) | |
| AA | CAR (MabCAR3_ACA-4 and ACA-1) and ω-TA | 59.4 | 0.863 (this work) | |
| 6-ACA | CAR (MabCAR3_ACA-4) and ω-TA | 82.5 | 1.599 (this work) | |
*The production time recorded in the original text was “overnight,” which was calculated as 8 hours here.