Fig. 9. PFCE-C25 NEs induce immune memory.

(A) Flow cytometry results showing the percentages of T_CM cells (CD8⁺ CD44^high CD62L^high) and T_EM cells (CD8⁺ CD44^high CD62L^low) in C25 cyclic peptide– and PFCE-C25 NE–treated group mice. The results were compared with those of the untreated group. (B) Regimen used for tumor rechallenge and corresponding statistics. (C) Representative H&E staining results of lungs from naïve LLC and rechallenge LLC groups. Scale bar, 20 μm. (D) Confocal fluorescence microscopy results for CD8⁺ CD44⁺ T cells in the TDLNs of PFCE NE–, PFCE-C25 NE–, and C25-treated groups. Scale bar, 100 μm. The data are presented as the mean ± SD.