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Cell binding studies evaluating [89Zr]Zr-DFO-2Rs15d. (A) Cell binding and specificity studies show [89Zr]Zr-DFO-2Rs15d has specific binding to HER2 while binding to a different epitope than trastuzumab. (n = 8 for each group) (B) Internalization studies showed a steady increase of cell-associated [89Zr]Zr-DFO-2Rs15d internalized over 72 h. (n = 6 for each group) **p < 0.01 and ****p < 0.0001.
