FIG. 6.

Cif activation requires caspases. Aliquots (100 μg) of S-100 cytosolic extract from Bcl-2:HL-60 cells were treated with 100 ng of caspase 3, caspase 7, or caspase 8 in the absence (lanes 1) or presence (lanes 2) of 10 μM Ac-DEVD-CHO at 37°C for 60 min. Alternatively, following caspase treatment, 10 μM Ac-DEVD-CHO was added to the samples (lanes 3). The samples were then incubated with HL-60 mitochondria for 15 min at 37°C and processed as described in the legend to Fig. 5A.