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Specific PI(4,5)P2-stimulated GTPase activities of wild-type (wt) and mutant dynamins as a function of dynamin concentration. Specific activities were calculated by dividing moles of Pi released per minute by the dynamin concentration in each assay. Activities in the absence of PI(4,5)P2 were subtracted from each data point. Experiments were performed in buffer A containing 0.1 M NaCl. The PI(4,5)P2 concentration was 5 μM.
