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eIF6 depletion results in a decrease in 60S subunits levels. Total ribosomes were isolated from strains W303α and KSY603 after 10 h of growth in either YPD or YPGal, dissociated into 40S and 60S ribosomal subunits, and sedimented through 15 to 40% sucrose gradients as described in Materials and Methods.
