FIG. 8.

Translation of luciferase mRNA in cell extracts. Translation cell extracts were prepared from W303α (TIF6) or KSY603 (GAL10::UbTIF6) cells grown in glucose-containing medium for 11 h as described in Materials and Methods. Where indicated, 2 μg of capped luciferase mRNA was added to 50-μl reaction mixtures along with all the other components necessary for in vitro translation. The reactions were carried out at 25°C. At the indicated times, an aliquot of each reaction mixture was assayed for luciferase activity as described by Russel et al. (25).