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Cellular internalization of DAP in HeLa cells. The green fluorescence was emitted from DAP, and blue from Hoechst33342. (a) Incubation time of 30 min (b) 1 h (scale bar: 10 µm); Cytotoxicity measurement by MTT assay. (c) HeLa and (d) HEY A8 cells were treated with DAP for 24 h; (e) Fluorescence emission spectra of DAP (0.002 M) in the presence of increasing [DNA] (1.24 × 10–6–1.15 × 10–5 M). (f) Change in color of DAP in presence and absence of DNA.
