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. 2024 Nov 29;56(1):7. doi: 10.1007/s10735-024-10312-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — Targeting circVAPA alleviates inflammatory damages in the pulmonary tissues of ARDS mouse model

ARDS model was established by pulmonary instillation of LPS through the trachea in mice. Animals were assigned into 4 groups: sham group: mice treated with sterile saline; Model group: mice stimulated with LPS as the ARDS model; Model + sh-NC group: model group administrated with lentivirus carrying control shRNA. Model + sh-circVAPA group: model group administrated with lentivirus carrying circVAPA shRNA. (A) qRT-PCR analysis of circVAPA, Sirt1 and miR-212-3p levels in the BALF samples. (B) ELISA analysis of inflammatory cytokines in the BALF samples. (C) MPO activities detection in the BALF samples. (D) ROS level measurement in the BALF samples. (E) Flow cytometry analysis of M1 (CD86) and M2 (CD206) marker expression in macrophages isolated from the BALF samples. (F) H&E staining in the pulmonary tissues. (G) Summary of wet/dry ratios of lung tissues. (H) Quantification of protein concentrations in the BALF samples. n = 6 animals in each group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001