FIG. 6.

Tumor-derived p53 mutants reduce p73α-induced apoptosis. H1299 cells were transiently cotransfected with wild-type p53, p53R175H, p53R248W, and p73α expression plasmids, singly or in combination as indicated, and a GFP expression plasmid. After 72 h, the morphology of the cells was observed under an epifluorescence microscope. (B) Representative apoptotic morphology of cells overexpressing p73α compared to control cells in panel A transfected with an empty CMV expression plasmid. (C) Results represented as percentage of apoptotic cells over 500 GFP-stained cells in a 10-cm-diameter plate (mean of a typical experiment performed with triplicates cultures; bars indicate standard deviations).