Fig. 2.

KLF4 knockdown aggravates DSS- and TNBS-induced acute colitis. A, B The body weight (A) and disease activity index (DAI) (B) of DSS-challenged mice after treatment with shKLF4-Adv. C, D Representative images of mouse colons (C) and colon length (D) of DSS-challenged mice after treatment with shKLF4-Adv. E Representative images of H&E staining of colon tissues from DSS-challenged mice after treatment with shKLF4-Adv. Scale bars, 500 μm (top) and 250 μm (bottom). F Summary of the histological scores of DSS-challenged mice after treatment with shKLF4-Adv. G Representative colonic AB-PAS images of DSS-challenged mice after treatment with shKLF4-Adv. Scale bars, 500 μm (top) and 250 μm (bottom). H The body weights of TNBS-challenged mice after treatment with shKLF4-Adv. I, J Representative images of mouse colons (I) and colon length (J) of TNBS-challenged mice after treatment with shKLF4-Adv. K Representative images of H&E staining of colon tissues from TNBS-challenged mice after treatment with shKLF4-Adv. Scale bars, 500 μm (top) and 250 μm (bottom). L Summary of the histological scores of DSS-challenged mice after treatment with shKLF4-Adv. M Representative colonic AB-PAS images of TNBS-challenged mice after treatment with shKLF4-Adv. Scale bars, 500 μm (top) and 250 μm (bottom). WT + water (n = 6), WT + DSS (n = 8), control-Adv + DSS (n = 5), shKLF4-Adv + DSS (n = 10), WT + Alcohol (n = 6), WT + TNBS (n = 8), control-Adv + TNBS (n = 5), and shKLF4-Adv + TNBS (n = 10). The data are expressed as the mean ± SD. ns, not significant, ** p < 0.01, *** p < 0.001