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. 2024 Nov 29;81(1):470. doi: 10.1007/s00018-024-05514-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 7 — m6A modification decreased KLF4 expression via YTHDF2. A ALKBH5 overexpression elevated the expression of KLF4 in Caco-2 cells at both the mRNA and protein levels. B Knockdown of ALKBH5 reduced the expression of KLF4 in Caco-2 cells at both the mRNA and protein levels. C The mRNA stability of KLF4 in Caco-2 cells after transfection with ALKBH5 siRNAs or overexpression plasmids was measured by RT‒qPCR. D The relative mRNA levels of YTHDF1, YTHDF2, YTHDF3 and KLF4 in Caco-2 cells after transfection with YTHDF1, YTHDF2 or YTHDF3 siRNAs. E The protein expression of YTHDF2 and KLF4 in Caco-2 cells after transfection with YTHDF2 siRNAs. F The mRNA stability of KLF4 in Caco-2 cells after transfection with YTHDF2 siRNAs was measured by RT‒qPCR. G RIP-qPCR analysis showed that the YTHDF2 protein bound to KLF4 mRNA. H, I mRNA (H) and protein (I) expression levels of KLF4 in Caco-2 cells after cotransfection with YTHDF2 siRNAs and ALKBH5 overexpression plasmids. The data are expressed as the mean ± SD. ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001