Fig. 8.

APTO-253 exhibits a synergistic effect with mesalazine in the preservation of IEB function. A KLF4 protein expression in Caco-2 cells co-stimulated with APTO-253 and mesalazine. B, C TEER (B) and FD4 (C) permeability were measured in the KLF4 knockdown Caco-2 monolayer after costimulation with TNF-α/IFN-γ (100 ng/ml) and APTO-253. D The protein expression of Occludin, ZO-1, Claudin-4 and E-cadherin in Caco-2 cells cotreated with KLF4 siRNA, TNF-α/IFN-γ (100 ng/ml) and APTO-253. E, F TEER (E) and FD4 permeability (F) were measured in Caco-2 cell monolayers cotreated with mesalazine, TNF-α/IFN-γ (100 ng/ml) and APTO-253. G The protein expression of Occludin, ZO-1, Claudin-4 and E-cadherin in Caco-2 cells cotreated with mesalazine, TNF-α/IFN-γ (100 ng/ml) and APTO-253. H The m6A modification of KLF4 mRNA in Caco-2 cells after APTO-253 and mesalazine co-stimulation. I The protein expression of METTL3, ALKBH5 and YTHDF2 in Caco-2 cells co-treated with mesalazine and APTO-253.The data are expressed as the mean ± SD. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001