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IAAP photoaffinity labeling in the presence of various concentrations of vinblastine. IAAP labeling was carried out as described above in the presence of vinblastine at concentrations of 0, 0.1, 0.3, 1, and 3 μM. After immunoprecipitation and SDS-PAGE separation, the radioactivity associated with P-gp was measured and quantitated by using the STORM system. The normalized IAAP labeling is shown. The relative IAAP labeling in the absence of vinblastine is shown as 100%.
