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. 2024 Nov 30;12(23):e70149. doi: 10.14814/phy2.70149

FIGURE 2.

FIGURE 2

NBP activates Sirt1/Nrf2 signaling pathway in I/R‐induced skeletal muscle injury. (a) Western blot analysis in gastrocnemius muscle indicates the effects of NBP on Sirt1, Nrf2 expression. Data are presented as relative fold change to the control group (set to 1). n = 6 per group. (b–d) H&E staining and immunofluorescence staining of the gastrocnemius muscle indicate the extent of muscle fiber injury and changes in muscle fiber cross‐sectional area across different groups after EX527 treatment. n = 8 per group. Scale bar = 100 μm. A microscope with a 40× objective was used to capture the images. (e–g) Western blot analysis in gastrocnemius muscle indicates the effects of 4‐HNE, Sirt1, Nrf2, Myog and MyoD expressions after EX527 treatment. Data are presented as relative fold change to the I/R group. n = 6 per group.