Figure 2.

CPT, DOX, and ETO augment AAV transduction in IMR90 cells

Contact-inhibited IMR90 cells were treated overnight with either medium (no drug control, green circles), 0.1 μM CPT (blue squares), 300 μM DOX (maroon upward triangles), or 100 μM of ETO (pink downward triangles) before drug washout. Treated cells were transduced with AAV-GFP and cultured in the absence of topoisomerase drugs. Over the first 120 h post transduction, phase and green fluorescence live cell images were taken every 12 h using the Incucyte S3 and 25 images were taken per well. The Incucyte analysis software was used to quantify GFP-positive foci, with the reported count taking an average across the 25 images. Data displayed are the values for each biological replicate (n = 4) at 96 h post transduction with the mean displayed as a horizontal bar. Fold changes were calculated by comparing the means of each drug condition. Data were analyzed using one-way ANOVA with Dunnett’s multiple comparison test for each drug against the control cells. ns, no significance; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.