FIGURE 1.

S1PR1 antagonism attenuates mechano‐allodynia. (A–D) Naive C57BL6/J mice were intraperitoneally injected with paclitaxel (2 mg/kg) or vehicle on four alternate days (0, 2, 4, 6). FTY720 (0.3 mg/kg) was orally administered concurrently with paclitaxel. Mechano‐allodynia (A) and cold‐allodynia (B) were measured in each paw on day 15. n = 3–4 mice per group. (C and D) FTY720 suppressed paclitaxel‐induced astrocyte reactivation in the DHSC. (C) Representative images (20X) of GFAP immunostained (green) DHSCs from mice treated with vehicle, paclitaxel, or paclitaxel +FTY720‐treated mice. Scale bar: 100 μm. (D) Quantitation of GFAP staining plotted as integrated density per field. n = 3 mice per group. Fifteen to sixteen images per group were acquired and quantified. Data are mean ± SEM. *p < .05 **p < .01, ****p < .0001. One‐way analysis of variance test followed by Tukey's multiple comparison test.