FIGURE 2.

FTY720 suppresses paclitaxel induced Sparcl1 expression in naïve mice. Mice were treated with vehicle, paclitaxel without or with FTY720, as described in Figure 1. (A) Representative confocal images of the dorsal horn spinal cord area immunostained for GFAP (green) and Sparcl1 (red). (B) Quantification of Sparcl1 fluorescence intensity plotted as integrated density per field. n = 3 mice per group. Fifteen to sixteen images per group were acquired and quantified. (C) Pearson's correlation coefficient of colocalization between GFAP and Sparcl1. n = 2 mice per group. Fifteen images per mouse were acquired and quantified. Data are expressed as mean ± SEM, ***p < .001, ****p < .0001. One‐way analysis of variance test followed by Tukey's multiple comparisons test.