FIGURE 3.

FTY720 suppresses paclitaxel‐induced excitatory synapses in naïve mice. Mice were treated with vehicle, paclitaxel without or with FTY720, as described in Figure 1. (A) Representative confocal images of the lamina I‐II area immunostained for VGluT2 (red) and PSD95 (green). Boxed area is zoomed in and shown in the lower panel. Excitatory synapses (arrow) are visualized as colocalized puncta (yellow) between VGluT2 and PSD95 puncta. Quantification of excitatory synapses (B), presynaptic terminal VGluT2 (C), and postsynaptic site PSD95 (D) as fold change compared to vehicle group. n = 3 mice per group. Eighteen images per group were acquired and quantified. Data are expressed as means ± SEM, ***p < .001. One‐way analysis of variance test followed by Tukey's multiple comparisons test.