FIGURE 6.

FTY720 suppresses paclitaxel‐induced astrocyte activation and Sparcl1 expression. E0771.LMB breast cancer cells were implanted in the mammary fat pads of female mice and 12 days later, mice were treated with vehicle, FTY720 (gavage 0.3 mg/kg), paclitaxel (i.p. 10 mg/kg), or both as described in Figure 4A. n = 4 mice per group. Lumbar DHSC was harvested from these mice on day 30. (A) Representative confocal images of the DHSC area immunostained for GFAP (green) and Sparcl1 (red). Quantification of GFAP (B) and Sparcl1 (C) fluorescence intensity plotted as integrated density per field. n = 4 mice per group. Nineteen to twenty‐six images per group were acquired and quantified. Data are expressed as means ± SEM, *p < .05, ***p < .001, ****p < .0001. One‐way analysis of variance test followed by Tukey's multiple comparisons test.