FIGURE 7.

FTY720 suppresses paclitaxel‐induced excitatory synapses. E0771.LMB breast cancer cells were implanted in the mammary fat pads of female mice and 12 days later, mice were treated with vehicle, FTY720 (gavage 0.3 mg/kg), paclitaxel (IP 10 mg/kg), or both (n = 4) as described in Figure 4A. Lumbar DHSC was harvested from these mice on day 30. (A) Representative confocal images of the lamina I‐II areas immunostained for VGluT2 (red) and PSD95 (green). Boxed area is zoomed in and shown in the lower panel. Excitatory synapses (arrow) are visualized as colocalized puncta (yellow) between VGluT2 and PSD95 puncta. Quantification of excitatory synapses (B), presynaptic terminal VGluT2 (C), and postsynaptic site PSD95 (D) as fold change compared to vehicle group. n = 4 mice per group. Sixteen images per group were acquired and quantified. Data are expressed as means ± SEM, *p < .05, **p < .01. One‐way analysis of variance test followed by Tukey's multiple comparisons test.