FIG. 11.

Tyrosine phosphorylation of Rlk-GFP in Jurkat cells. Cells were electroporated with either WT Rlk-GFP or AUG2Rlk-GFP expressing only the short form of Rlk. Twenty-four hours later, cells were stimulated with anti-CD3 antibody OKT3 for the indicated times (in minutes). Cells were lysed and Rlk was immunoprecipitated. Immunoprecipitated Rlk was immunoblotted with antiphosphotyrosine (4G10) (top) or anti-Rlk (bottom).