FIG. 8.

WT Rlk is modified by palmitoylation. Mutation of the cysteine string abolishes [³H]palmitate labeling. 293T cells were transfected with cDNAs encoding Fyn (positive control, lane 1), a cysteine mutant of Rlk-GFP (lane 2), WT Rlk (lane 3), or control vector alone (lane 4). Twenty-four hours after transfection, cells were labeled with [³H]palmitate for 2 h, washed, and lysed, and Rlk or Fyn was immunoprecipitated as described in the text. (Top) Samples were analyzed by SDS–10% PAGE and visualized by fluorography. (Bottom) Immunoblot (IB) for Rlk.