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Performance evaluation of HFPN and its impact on ROS production. Note: (A) The experimental procedure; (B) Absorbance spectra of MB in different cell groups; (C) Changes in absorbance values of MB in different cell groups; (D) Visualization of ROS in MDA-MB-231 cells in different groups using CLSM, with red fluorescence (DHE) representing ROS. *** indicates a significant difference between the two groups with P < 0.001. The experiment was repeated three times
