Fig. 1.

YZ-836P was identified as a PRMT5 PROTAC in TNBC. (A) PRMT5 and KLF5 protein expression levels in breast cancer cell lines and immortalized breast epithelial cell lines were detected by WB. (B) The cytotoxic effects of 49 compounds on HCC1806 and HCC1937 cells were assessed by the SRB assay (48 h, 4 µM). (C) Evaluation the effects of candidate compounds (48 h, 4 µM) on the protein level of PRMT5 and KLF5 by WB. (D) YZ-836P reduced PRMT5 and KLF5 protein levels in a concentration-dependent manner (48 h) in both HCC1806 and HCC1937 cell lines, as determined by WB. (E) YZ-836P could reduce PRMT5 and KLF5 proteins in a time-dependent manner (48 h, 4 µM). (F) The molecular formula and molecular weight of YZ-836P and YZ-850A