Fig. 2.

Ectopic expression of PAX6 inhibits AIG and invasion in a constitutive expression system in liver cancer cells. A The endogenous protein expression of PAX6 in liver cancer cell lines was determined by western blotting using an anti-PAX6 antibody. B Stable overexpression of pLAS2.1-PAX6 was established in different liver cancer cell lines and confirmed by western blotting. β-Actin was used as an internal control. C Cell proliferation (MTS) assays D and AIG assays were performed in HAT22T, SK-Hep1 and Huh7 cells expressing PAX6. E Matrigel invasion assays were performed in HAT22T, SK-Hep1 and Huh7 cells expressing PAX6. The data are expressed as the mean ± SE. Significant differences were determined using the Mann‒Whitney U test. ** p < 0.01, *** p < 0.001