Fig. 6.

PAX6 knockout in HepG2 cells promoted tumor growth in xenograft NOD/SCID mice. A A diagram demonstrating the time schedule of the xenograft experiments. B The detailed operations of xenograft mice are illustrated. PAX6 knockout HepG2 cells or vector-HepG2 cells were subcutaneously injected into the left and right flanks of NOD/SCID mice. C The tumor growth curves of PAX6 knockout cells were compared with those of vector-only cells. D The weights of the PAX6 knockout tumors were compared to those of the vector-treated tumors. The data are expressed as the mean ± SE. Significant differences were determined using the unpaired two-tailed t test. ** p < 0.01