Fig. 8.

Knockdown of CDH1 in PAX6-expressing HCC cells repressed the tumor suppressive function of PAX6. The expression of CDH1 in PAX6-expressing HCC cells (HA22T and Huh7) transfected with shCtrl or CDH1 shRNAs (shRNA CDH1-1 and shRNA CDH1-2) was analyzed by western blotting analysis. β-Actin was used as an internal control. B Colony formation and C Matrigel invasion assays were used to analyze the malignant phenotypes. The data are presented as the mean ± SE. Statistical significance was calculated with the Mann‒Whitney U test. * p < 0.05 and ** p < 0.01